On Thursday 29 April 2021, starting at 2.30 pm, on the “Microsoft Teams” platform, another Lecture of the Jean Monnet EUWEB Module took place, dedicated to Investigative Techniques, Evidence Gathering and “Proliferation” of Databases in Judicial and Police Cooperation. The Lecture started with the introduction by Prof. Gaspare Dalia (The Exchange of Information as a Tool for Judicial and Police Cooperation: Current Challenges and Future Perspectives) and by Profs. Teresa Russo and Anna Oriolo, which were followed by the speeches of Prof. Paolo Troisi (Principle of Availability, Horizontal Cooperation and PNR Data Exchange), Prof. Donatello Cimadomo (The New IT Frontiers of Judicial Cooperation. ECRIS Between Technical Rules and Practical Needs), Dr. Lucia Iodice (ECRIS-TCN. Artificial Intelligence, Police Investigations and Respect of Data Protection Rules), Prof. Pietro Milazzo (The Proliferation of Police Databases and the European Regulation on Personal Data: Perspectives and Limits of the Directive (EU) 2016/680), by Dr. Giorgio Butini (Databases and Investigative Techniques in International Police Cooperation: Key Performance Indicators of the Approximation to European Union?) and by Dr. Gezim Spahiu (Mutual Legal Assistance Instruments in Albania). The works were closed by the final debate.